Molecular Biology Tools Websites

The restriction enzyme database. Maintained by the New England Biolabs, USA.

Provides restriction analysis, silent mutation scanning and SNP-RFLP analysis. Allows for utilization of all commercially available type II restriction enzymes.

Finds restriction enzyme cleavage sites in linear and circular DNA sequences. Provides also a virtual digest function.

Displays the codon quality in two different ways, codon usage frequency or relative adaptiveness values. FAQ included.

Offers server generated plasmid maps and image files, from input DNA sequences up to 20,000 bp. Includes citation, and downloadable source code. Maintained by the University of Alberta, USA.

Provides a DNA sequence analysis to find restriction enzymes sites. Finds also non-overlapping open reading frames using the E.coli genetic code.

Converts amino acid and nucleotide sequence data formats including FASTA, GenBank, Phylip and others. Maintained by NIH, USA.

Provides reverse complement sequence for DNA or RNA primer strands, self complementarity check, physical and thermodynamic constants, melting temperature (Tm). Includes calculation formulas.

Provides primers specific to the PCR template sequence. Includes primer pair specificity checking against a selected database.

A Java application that facilitates the identification of potential ORFs, based on provided DNA sequence and probable ORF length.

Identifies all ORFs using the standard or alternative genetic codes. Provides an interactive, graphical interface and allow for saving of a deduced amino acid sequence in various formats. Allows searching against the sequence database using the BLAST server.

Provides restriction enzyme digest analysis of the input DNA sequence. Results are presented graphically as a "virtual gel".

DNA restriction enzyme analysis. Provides a number of hits per enzyme and a list of non-cutters. Allows for selection of the enzymes and parameters.

Silent mutation restriction enzyme scan. Finds new restriction sites to insert (mutate) with no translation change.

Determines the number and highlights a rare E. coli codons in a provided DNA sequence. Maintained by UCLA, USA.

Provides graphical and numerical representation of the codon adaptation index, frequency distribution and GC content of the DNA sequence. Maintained by the GenScript, USA.