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PCR Websites

Polymerase Chain Reaction

Techniques used for replication of a single strand of DNA to provide sufficient quantity for other applications.

- Category ID : 417082
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Ingenetix GmbH

Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna, Austria.
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Roe Laboratory Protocols

Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
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The Web Guide of PCR

List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
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HiFi DNA

HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
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PCR Protocols

Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
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Thermostable DNA Polymerases

Discussion of their origin and briefly their properties. From the web site of Colorado State University.
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Which DNA Marker for Which Purpose?

Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen.
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Single Tube Confirmation PCR Protocol

For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
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GenHunter

Manufacturer of material for differential display PCR in Nashville, Tenn USA.
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Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation

Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
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T-DNA Generated Enhancer Traps in Arabidopsis

Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.
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PCR Amplification of cDNA Segments by 2 Stage Nested PCR

Protocol from the method database of NIH, USA.
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PCR-ELISA and Related

P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA.
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Nematode ITS1 Size Variation

Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.
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Attotron Biosensor Corporation

Research and development company for development of biosensors and related products for the research and educational markets.
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Protocols Online: PCR Protocols

Extensive collection of PCR protocols and methods from Protocol On Line.
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Kary B. Mullis - Autobiography

The originator of PCR, from the Nobel e-museum web site.
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Reference in PCR

Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
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GeneOhm Sciences

Tests on group B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
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Principle of PCR

Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
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PCR Animated

Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
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PCR Gateway

A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
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BioRad, Amplification, PCR

Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.
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PCR Guru

A downloadable textbook on PCR setup and optimization, not free.
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The PCR Encyclopedia

Describes plans for a free encyclopedia dedicated to the polymerase chain reaction (PCR).
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Primerfox

Free online tool for generation of PCR primers.
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PrimerDigital

International biotechnology company specialized in a design service for PCR primers and probes, PCR-based technology development, projects for development of polymorphism and software development.
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Inverse PCR for Use with Snyder mTn-lacZ/LEU2-based Mutagenesis

Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
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Inverse PCR Protocol

Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
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PCR Protocol

Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
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Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)

Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.

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